著書

マリエル生命科学

大学生用の生命科学の教科書です。生理学的な内容を豊富に盛り込んでいます。第11章（遺伝子工学）、第18章（免疫系）を翻訳しています。また全体の章の監訳も行っています。

Live Cell Imaging

蛍光タンパク質センサーの開発手法を執筆しています。これを読めば、自在に作出できるようになります。

みんなの生命科学　第2版

高校時代に生物を履修していない大学生用の教科書です。第3章（生命を理解するための科学技術）、第4章（ヒトの誕生と成長）、第5章（ヒトの寿命と死）、第13章（外敵から身を守るしくみ）、第14章（社会性を生み出す脳）を執筆しています。

図説生物学　Biology Illustrated

「膜電位発生の仕組み、イオンチャネル」「神経細胞の構造、様々な形態・シナプスと興奮伝達の制御」を執筆しています。

学術論文(最近5年間)

Mina Horikoshi, Kazuki Harada, Saki Tsuno, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto, Shin Terada and Takashi Tsuboi. Distinct lactate metabolism between hepatocytes and myotubes revealed by live cell imaging with genetically encoded indicators. Biochem Biophys Res Commun 694, 149416, 2024.

Saki Tsuno, Kazuki Harada, Mina Horikoshi, Marie Mita, Tetsuya Kitaguchi, Masami Yokota Hirai, Mitsuharu Matsumoto and Takashi Tsuboi. Mitochondrial ATP concentration decreases immediately after glucose administration to glucose-deprived hepatocytes. FEBS Open Bio 14, 79-95, 2024. 

Junfeng Wang, Eijiro Maeda, Yuki Tsujimura, Takaya Abe, Hiroshi Kiyonari, Tetsuya Kitaguchi, Hideo Yokota and Takeo Matsumoto. In situ FRET measurement of cellular tension using CLSMs: Reporter mice expressing an actinin tension sensor. Sci Rep 13, 22729, 2023.

Kana Sasamoto, Takanobu Yasuda, Bo Zhu, Hiroshi Ueda and Tetsuya Kitaguchi. Efficient and rapid linker optimization with heterodimeric coiled coils improves the response of fluorescent biosensors comprising antibody and protein M. Analyst 148, 5843-5850, 2023.

Bo Zhu, Zhe Du, Yancen Dai, Tetsuya Kitaguchi, Sebastian Behrens and Burckhard Seelig. Nanodroplet-based reagent delivery into water-in-fluorinated-oil droplets. Biosensors 13, 768, 2023.

Sohei Hiasa, Takeru Fujimori, Saki Aiki, Hiroshi Ueda, Takashi Tsuboi and Tetsuya Kitaguchi. Development of green fluorescent protein-based cAMP indicators for covering a wide range of cAMP concentrations. RSC Adv 13, 15514-15520, 2023.

Yoshihiro Ito, Ryuichi Sasaki, Sayaka Asari, Takanobu Yasuda, Hiroshi Ueda and Tetsuya Kitaguchi. Efficient microfluidic screening method using a fluorescent immunosensor for recombinant protein secretions. Small 19, 2207943, 2023.

Abdul Q. Rani, Bo Zhu, Hiroshi Ueda and Tetsuya Kitaguchi. Recent progress in homogeneous immunosensors based on fluorescence or bioluminescence using antibody engineering. Analyst 148, 1422-1429, 2023. (Review)

Donnell White III, Lothar Lauterboeck, Parnia Mobasheran, Tetsuya Kitaguchi, Antoine H. Chaanine and Qinglin Yang. Real-time visualization of cytosolic and mitochondrial ATP dynamics in response to metabolic stress in cultured cells. Cells 12, 695, 2023.

Kazuki Harada, Maoko Takashima, Tetsuya Kitaguchi and Takashi Tsuboi. Time-dependent shift in docking dynamics of glucagon-like peptide-granules from enteroendocrine L cell line GLUTag cells. FEBS Lett 597, 657-671, 2023.

Jiulong Su, Bo Zhu, Akihito Inoue, Hiroyuki Oyama, Izumi Morita, Jinhua Dong, Takanobu Yasuda, Yoshiko Sugita-Konishi, Tetsuya Kitaguchi, Norihiro Kobayashi, Shiro Miyake and Hiroshi Ueda. The Patrol Yeast: a new biosensor armed with antibody-receptor chimera detecting a range of toxic substances associated with food poisoning. Biosens Bioelectron 219, 114793, 2023.

Bo Zhu, Cheng Qian, Haoxuan Tang, Tetsuya Kitaguchi and Hiroshi Ueda. Creating a thermostable beta-glucuronidase switch for homogeneous immunoassay by disruption of conserved salt bridges at diagonal interfaces. Biochemistry 62, 309-317, 2023.

Bo Zhu, Nobuyuki Nosaka, Shuji Kanamaru, Jinhua Dong, Yancen Dai, Akihito Inoue, Yinghui Yang, Kaori Kobayashi, Tetsuya Kitaguchi, Hiroshi Iwasaki, Ryuji Koike, Kenji Wakabayashi and Hiroshi Ueda. Rapid and sensitive SARS-CoV-2 detection using a fluorescent immunosensor Quenchbody with crowding agents. Analyst 147, 4971-4979, 2022.

Fumiya Uefune, Toru Aonishi, Tetsuya Kitaguchi, Harumi Takahashi, Susumu Seino, Daisuke Sakano and Shoen Kume. Dopamine negatively regulates insulin secretion through activation of D1-D2 receptor heteromer. Diabetes 71, 1946-1961, 2022.

Mai Takizawa, Yuri Osuga, Rika Ishida, Marie Mita, Kazuki Harada, Hiroshi Ueda, Tetsuya Kitaguchi and Takashi Tsuboi. Development of a red fluorescent protein-based cGMP indicator applicable for live-cell imaging. Commun Biol 5, 833, 2022.

Yancen Dai, Yuko Sato, Bo Zhu, Tetsuya Kitaguchi, Hiroshi Kimura, Farid J. Ghadessy and Hiroshi Ueda. Intra Q-body: an antibody-based fluorogenic probe for intracellular proteins that allows live cell imaging and sorting. Chem Sci 13, 9739-9748, 2022.

Ferdinandus, Madoka Suzuki, Yoshie Harada, Cong Quang Vu, Satya Ranjan Sarker, Shin’ichi Ishiwata, Tetsuya Kitaguchi and Satoshi Arai. Modulation of local cellular activities using a photothermal dye-based subcellular-sized heat spot. ACS Nano 16, 9004-9018, 2022.

Marie Mita, Izumi Sugawara, Kazuki Harada, Motoki Ito, Mai Takizawa, Kentaro Ishida, Hiroshi Ueda, Tetsuya Kitaguchi and Takashi Tsuboi. Single fluorescent protein-based red glucose indicators visualize intracellular glucose dynamics. Cell Chem Biol,  29, 98-108.E4, 2022. 

Akihito Inoue, Takanobu Yasuda, Bo Zhu, Tetsuya Kitaguchi, Akikazu Murakami and Hiroshi Ueda. Evaluation and selection of potent fluorescent immunosensors by combining fluorescent peptide and nanobodies displayed on yeast surface. Sci Rep 11, 22590, 2021.

Keijun Kakihara, Kengo Asamizu, Kei Moritsugu, Masahide Kubo, Tetsuya Kitaguchi, Akinori Endo, Akinori Kidera, Mitsunori Ikeguchi, Akira Kato, Masayuki Komada and Toshiaki Fukushima. Molecular basis of ubiquitin-specific protease 8 autoinhibition by the WW-like domain. Commun Biol 4, 1272, 2021.

Xuerao Ning, Takanobu Yasuda, Tetsuya Kitaguchi and Hiroshi Ueda. Construction of fluorescent biosensor Q-body for detecting His-tagged recombinant proteins produced in bioprocess. Sensors 21, 4993, 2021.

Takanobu Yasuda, Akihito Inoue, Tetsuya Kitaguchi and Hiroshi Ueda. Rapid construction of fluorescence quenching-based immunosensor Q-bodies using α-helical coiled-coil peptides. Chem Comm 57, 8206-8209, 2021.

Yuki Ohmuro-Matsuyama, Tetsuya Kitaguchi, Hiroshi Kimura and Hiroshi Ueda. A simple fluorogenic cellular assay for histone deacetylase inhibitors based on split-YFP and intrabodies. ACS Omega 6, 10039-10046, 2021.

Kazuki Harada, Takami Chihara, Yuki Hayasaka, Marie Mita, Mai Takizawa, Kentaro Ishida, Mary Arai, Saki Tsuno, Mitsuharu Matsumoto, Takeshi Ishihara, Hiroshi Ueda, Tetsuya Kitaguchi and Takashi Tsuboi. Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging. Sci Rep 10, 19562, 2020.

Akihito Inoue, Yuki Ohmuro-Matsuyama, Tetsuya Kitaguchi and Hiroshi Ueda. Creation of a nanobody-based fluorescent immunosensor mini Q-body for rapid signal-on detection of small hapten methotrexate. ACS Sens 5, 3457-3464, 2020.

Jinhua Dong, Chihiro Miyake, Takanobu Yasuda, Hiroyuki Oyama, Izumi Morita, Tomoya Tsukahara, Masaki Takahashi, Hee-Jin Jeong, Tetsuya Kitaguchi, Norihiro Kobayashi and Hiroshi Ueda. PM Q-probe: a fluorescent binding protein that converts many antibodies to a fluorescent biosensor. Biosens Bioelectron, 165, 112425, 2020.

Chungyong Han, Beom K. Choi, Seon-Hee Kim, Su-Jung Sim, Seongeun Han, Bomi Park, Yohei Tsuchiya, Masaki Takahashi, Young H. Kim, Hyeon-Seok Eom, Tetsuya Kitaguchi, Hiroshi Ueda and Byoung S. Kwon. Polymorphic region-specific antibody for evaluation of affinity-associated profile of chimeric antigen receptor. Mol Ther Oncolytics 17, 293-305, 2020.

Yuki Oe, Xiaowen Wang, Tommaso Patriarchi, Ayumu Konno, Katsuya Ozawa, Kazuko Yahagi, Hirokazu Hirai, Takashi Tsuboi, Tetsuya Kitaguchi, Lin Tian, Thomas J McHugh and Hajime Hirase. Distinct temporal integration of noradrenaline signaling by astrocytic second messengers during vigilance. Nat Commun 11, 471, 2020.

Takumi Nakamura, Kazuki Harada, Taichi Kamiya, Mai Takizawa, Jim Küppers, Kazuo Nakajima, Michael Gütschow, Tetsuya Kitaguchi, Kunihiro Ohta, Tadafumi Kato and Takashi Tsuboi. Glutamine-induced signaling pathways via amino acid receptors in enteroendocrine L cell lines. J Mol Endocrinol 64, 133-143, 2020.

Yasushi Kanai, Yuki Ohmuro-Matsuyama, Masami Tanioku, Shota Ushiba, Takao Ono, Koichi Inoue, Tetsuya Kitaguchi, Masahiko Kimura, Hiroshi Ueda and Kazuhiko Matsumoto. Graphene field effect transistor-based immunosensor for ultrasensitive noncompetitive detection of small antigens. ACS Sens 5, 24-28, 2020.

Jiulong Su, Tetsuya Kitaguchi, Yuki Ohmuro-Matsuyama, Theresa Seah, Farid J. Ghadessy, Shawn Hoon and Hiroshi Ueda. Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens. Sci Rep 9, 18189, 2019.

Chihiro Adachi, Naoto Kakinuma, Soohyun Jo, Takayuki Ishii, Yusuke Arai, Satoshi Arai, Tetsuya Kitaguchi, Sen Takeda and Takafumi Inoue. Sonic hedgehog enhances calcium oscillations in hippocampal astrocytes. J Biol Chem 294,  16034-16048, 2019.

Junfeng Wang, Shukei Sugita, Tatsuo Michiue, Takashi Tsuboi, Tetsuya Kitaguchi and Takeo Matsumoto. A novel FRET analysis method for a single actin stress fiber: Application to MC3T3-E1 cells during movement on a substrate. J Biorheol 33, 21-26, 2019.

Jiulong Su, Cyrus Beh, Yuki Ohmuro-Matsuyama, Tetsuya Kitaguchi, Shawn Hoon and Hiroshi Ueda. Creation of stable and strictly regulated enzyme switch for signal-on immunodetection of various small antigens. J Biosci Bioeng 128, 677-682, 2019.

Marie Mita, Motoki Ito, Kazuki Harada, Izumi Sugawara, Hiroshi Ueda, Takashi Tsuboi and Tetsuya Kitaguchi. Green fluorescent protein-based glucose indicators report glucose dynamics in living cells. Anal Chem 91, 4821-4830, 2019.