Our body is composed of trillions of cells, and It maintains its homeostasis by controlling many signaling molecules in the cells. To understand molecular mechanism of physiology and pathology in living organism, visualizing these signaling molecules and/or physiological events in living cells with high spatiotemporal resolution under optical microscope was required. Therefore, our lab challenges to develop genetically-encoded biosensors, and also generate technology to manipulate living cell from outside. To create these bioimaging tools, we ingeniously utilize fluorescent proteins, antibodies and enzymes.

We are developing two types of biosensors respond to variety of physiological signal in live cells and whole animal bodies by utilizing each strength and character. To accomplish the multi-color imaging, we focus on developing single fluorescent protein (FP)-based sensors. The molecular design of FRET-based sensors is well established than single FP-based sensors because of their developing history. Therefore, FRET-based sensors are developing for visualizing a novel phenomenon in live cells.

For more information about our research, Please check the web page below. 

●Flashbody (fluorescent protein-based sensor using antibody for molecular recognition)

●Glifon (glucose sensor based on fluorescent protein)